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KMID : 1023120110090040247
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Korean Journal of Aesthetic Society
2011 Volume.9 No. 4 p.247 ~ p.156
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Effect of Rosemary Extract on Antioxidative Activity and Melanogenesis in Cultured SK-MEL-3 Cells
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Oh Yong-Leol
Seo Young-Mi
Yang Hyun-Ok
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Abstract
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To evaluate the effect of Rosemary(Rosemarinus officinalis) extract on antioxidative activity and melanogenesis on cultured SK-MEL-3, the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, tyrosinase activity and a total amount of melanin were measured. In this study, SK-MEL-3 cells were treated with the media containing 1∼20μM, hydrogen peroxide(H2O2) for 4 hours. H2O2 significantly decreased cell viability compared with control and XTT50 value was determined at 20μM of H2O2. In the antioxidative effect of Rosemary extract, it showed DPPH-radical scavenging activity and SOD-like activity. While, in the melanogenic effect of Rosemary extract, it significantly decreased the tyrosinase activity and a total amount of melanin which were increased by H2O2. From above the results, it is suggested that Rosemary extract was effective in the protection of H2O2-induced cytotoxicity by antioxidative activity and inhibition of melanogenesis.
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KEYWORD
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Cell viability, Hydrogen peroxide, Cytotoxicity, Tyrosinase activity, Superoxide dismutase
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